文献类型：期刊文献

英文题名：The sulfated modification of polysaccharide from Poria cocos and its prebiotic effects on gut microbiota

作者：Ma, Linhua Zhang, Qifan Lai, Yong Long, Xiao Wang, Fang Feng, Bimin Guo, Xiurong Liu, Qiuyu Song, Can

第一作者：Ma, Linhua

通信作者：Song, C[1]

机构：[1]Southwest Med Univ, Sch Pharm, 319 Sect 3,Zhongshan Rd, Luzhou 646000, Sichuan, Peoples R China;[2]Guizhou Inst Technol, Sch Mat & Energy Engn, Guiyang, Peoples R China

第一机构：Southwest Med Univ, Sch Pharm, 319 Sect 3,Zhongshan Rd, Luzhou 646000, Sichuan, Peoples R China

通信机构：corresponding author), Southwest Med Univ, Sch Pharm, 319 Sect 3,Zhongshan Rd, Luzhou 646000, Sichuan, Peoples R China.

年份：2025

外文期刊名：JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE

收录：;Scopus(收录号：2-s2.0-105010653361);WOS:【SCI-EXPANDED(收录号:WOS:001525356600001)】；

基金：This work is supported by the Applied Basic Research Project of Southwest Medical University (2023QN032).

语种：英文

外文关键词：alkali-soluble polysaccharides; gut microbiota; Poria cocos; prebiotic; sulfated polysaccharides

摘要：BACKGROUNDPoria cocos is a renowned medicinal and edible macrofungus in China. Alkali-soluble polysaccharide from Poria cocos (PCY) is one of its main active components. However, the biological activity and utilization of PCY are constrained by its poor water solubility. Therefore, we modified PCY with chlorosulfonic acid-pyridine to improve its properties in this study. By comparing the results of solubility, antioxidant and hypoglycemic assays, we chose one derivative for further studies, which was named PCY-S2. We simulated in vitro the digestion and microbial fermentation of PCY-S2 using feces from volunteers and then gavaged it to mice to investigate its impacts on gut microbial modulation.RESULTSThe results showed that PCY-S2 could effectively regulate gut microbiota in fecal fermentation and animal experiments. In in vitro microbial fermentation, PCY-S2 upregulated the abundance of probiotic bacteria such as Streptococcus, Bifidobacterium and Lactococcus. In in vivo microbial studies, PCY-S2 downregulated the ratio of Firmicutes to Bacteroidota at the phylum level, significantly increasing the relative abundance of several beneficial microbiota such as unclassified_f_Muribaculacea, Prevotellaceae_UCG-001 and Alloprevotella at the genus level. Also, it promoted short-chain fatty acid production and reduced the levels of IL-6 and TNF-alpha.CONCLUSIONOverall, PCY-S2 is beneficial for regulating the composition and structure of gut microbiota, which provides a candidate for developing an effective functional prebiotic. (c) 2025 Society of Chemical Industry.