文献类型：期刊文献

英文题名：Sanggenon C Stimulates Osteoblastic Proliferation and Differentiation, Inhibits Osteoclastic Resorption, and Ameliorates Prednisone-Induced Osteoporosis in Zebrafish Model

作者：Wang, Huijuan Feng, Tingting Guo, Donggui Zhang, Min Chen, Lin Zhou, Ying

第一作者：Wang, Huijuan

通信作者：Zhou, Y[1];Zhou, Y[2]

机构：[1]Guizhou Univ, Guizhou Engn Res Ctr Exploitat & Utilizat Technol, Guiyang 550025, Guizhou, Peoples R China;[2]Guizhou Univ Chinese Med, Coll Pharm, Guiyang 550025, Guizhou, Peoples R China;[3]Guizhou Inst Technol, Sch Food & Pharmaceut Manufacture Engn, Guiyang 550003, Guizhou, Peoples R China

第一机构：Guizhou Univ, Guizhou Engn Res Ctr Exploitat & Utilizat Technol, Guiyang 550025, Guizhou, Peoples R China

通信机构：corresponding author), Guizhou Univ, Guizhou Engn Res Ctr Exploitat & Utilizat Technol, Guiyang 550025, Guizhou, Peoples R China;corresponding author), Guizhou Univ Chinese Med, Coll Pharm, Guiyang 550025, Guizhou, Peoples R China.

年份：2018

卷号：23

期号：9

外文期刊名：MOLECULES

收录：;Scopus(收录号：2-s2.0-85053637374);WOS:【SCI-EXPANDED(收录号:WOS:000447365100254)】；

基金：This work was supported by the National Natural Science Foundation of China (No. 81603390), the Science and Technology Major Project of Guizhou Province (No. [2015] 2002-3-4), the Science and Technology Planning Project of Guizhou Province (No. [2015]7100), and the Social Plan Project of Guizhou Province (No. [2016]2909).

语种：英文

外文关键词：Sanggenon C; osteoblast; osteoclast; osteoporosis; zebrafish

摘要：Sanggenon C (SC), which is a natural flavonoid found in the stem bark of Cortex Mori, has been discovered to have the antioxidant, anti-inflammatory, and antitumor properties. However, its effect in osteoporosis has not yet been reported. In this research, the effect of SC on the proliferation of MC3T3-E1 cells was evaluated by using the MTT assay. Alkaline phosphatase (ALP) activity and the mRNA expression of Runx2, Collagen I, OPG, and RANKL were examined. TRAP-positive cell counting and bone resorption pits were adopted to observe the effect of SC on the formation and function of osteoclasts. Next, the mRNA level of TRAP, CTSK, NFATc1, and TRAF6 of osteoclasts were measured by real-time qPCR. In addition, the anti-osteoporosis activity of SC in vivo was evaluated in the zebrafish model. Our study indicated that SC exhibited a significant stimulatory effect on MC3T3-E1 cell proliferation at 1 to 10 mu M and caused an increase in ALP activity at 0.3 to 10 mu M. It could upregulate the expression of Runx2, Collagen I, and increases the OPG/RANKL ratio. Furthermore, SC was found to inhibit the formation and function of osteoclasts, which is demonstrated by a lower number of TRAP-positive multinuclear cells and a fewer area of bone resorption pits compared to the control group. TRAP, CTSK, and NFATc1 were downregulated in 0.3 to 10 mu M SC treated groups. In addition, 3 to 10 mu M SC also inhibited the expression of TRAF6 mRNA. When prednisone-induced zebrafish was treated with 0.3, 1, 3, and 10 mu M SC, higher mineralization of vertebrate column was discovered in a dose-dependent pattern, which suggests that SC could reverse the bone loss of zebrafish caused by prednisone. In summary, these findings indicated that SC has the potential to prevent or treat osteoporosis.