文献类型：期刊文献

英文题名：Spatiotemporally Orchestrated Bone Healing: A Composite Scaffold System for Sequential Hemostasis and Osteogenesis

作者：Wang, Kaiyang Xia, Pengcheng Shao, Huifeng Wu, Qilin Yang, Linxin Liu, Guofeng Qiao, Tianhong Yao, Ke Lin, Xuanhe He, Jing Zou, Zhongfei Shen, Kangning Chen, Yuewei Sun, Yuan He, Yong

第一作者：Wang, Kaiyang

通信作者：Sun, Y[1];He, Y[1];He, Y[2];Sun, Y[3];He, Y[3];Sun, Y[4];He, Y[4];Chen, YW[5]

机构：[1]Zhejiang Univ, Affiliated Hosp 2, Hangzhou 310027, Peoples R China;[2]Xiamen Univ, Sch Med, Xiangan Hosp, Xiamen 361102, Fujian, Peoples R China;[3]Zhejiang Univ, Coll Mech Engn, Zhejiang Key Lab Addit Mfg Technol & Equipment, Hangzhou 310027, Peoples R China;[4]Sichuan Univ, State Key Lab Oral Dis, West China Hosp Stomatol, Chengdu 610041, Sichuan, Peoples R China;[5]Sichuan Univ, West China Hosp Stomatol, Natl Ctr Stomatol, Chengdu 610041, Sichuan, Peoples R China;[6]Sichuan Univ, West China Hosp Stomatol, Natl Clin Res Ctr Oral Dis, Chengdu 610041, Sichuan, Peoples R China;[7]Zhejiang Prov Peoples Hosp, Hangzhou Med Coll, Ctr Reprod Med, Dept Gynecol,Affiliated Peoples Hosp, Hangzhou 314408, Zhejiang, Peoples R China;[8]Hangzhou Dianzi Univ, Sch Mech Engn, Hangzhou 310018, Peoples R China;[9]Nanjing Med Univ, Nanjing Hosp 1, Dept Orthopaed, Nanjing 210006, Peoples R China;[10]Shanghai Univ, Natl Ctr Translat Med Shanghai, SHU Branch, Shanghai 200444, Peoples R China;[11]Guizhou Inst Technol, Sch Mech Engn, Guiyang 550003, Peoples R China;[12]Zhejiang Univ, State Key Lab Fluid Power & Mechatron Syst, Hangzhou 310027, Peoples R China;[13]Zhejiang Univ, Sch Mech Engn, Liangzhu Lab, Hangzhou 310027, Peoples R China;[14]Guizhou Univ, Sch Mech Engn, Guiyang 550025, Peoples R China

第一机构：Zhejiang Univ, Affiliated Hosp 2, Hangzhou 310027, Peoples R China

通信机构：corresponding author), Zhejiang Univ, Affiliated Hosp 2, Hangzhou 310027, Peoples R China;corresponding author), Zhejiang Univ, Coll Mech Engn, Zhejiang Key Lab Addit Mfg Technol & Equipment, Hangzhou 310027, Peoples R China;corresponding author), Zhejiang Univ, State Key Lab Fluid Power & Mechatron Syst, Hangzhou 310027, Peoples R China;corresponding author), Zhejiang Univ, Sch Mech Engn, Liangzhu Lab, Hangzhou 310027, Peoples R China;corresponding author), Guizhou Univ, Sch Mech Engn, Guiyang 550025, Peoples R China.

年份：2026

卷号：18

期号：7

起止页码：10948-10962

外文期刊名：ACS APPLIED MATERIALS & INTERFACES

收录：;EI(收录号：20260920158952);WOS:【SCI-EXPANDED(收录号:WOS:001691776800001)】；

基金：This work was sponsored by the National Key Research and Development Program of China (2024YFB4607700), the National Natural Science Foundation of China (52235007, T2121004, 52325504, 82301829) and Key R&D Program of Zhejiang (2024SSYS0027).

语种：英文

外文关键词：3D printing; directional freeze-casting; magnesium-dopedwollastonite; chitosan; bone regeneration

摘要：The instability of hematomas in open bone injuries presents a major barrier to effective healing. To address this issue, we developed a composite scaffold system designed to achieve temporally coordinated hematoma stabilization and osteogenic induction. The system comprises a 3D-printed rigid PCL + CSi-Mg framework integrated with a directionally frozen, highly ordered porous CS + CSi-Mg sponge. The composite scaffold demonstrated outstanding synergistic performance. The internal CS + CSi-Mg sponge, characterized by aligned pore channels and bioactive components (CS and Ca2+), facilitated rapid blood absorption and coagulation both in vitro and in vivo, achieving complete blood uptake within 10 s. Meanwhile, the external PCL + CSi-Mg framework provided mechanical strength comparable to that of cancellous bone (similar to 8.6 MPa). In vitro assays confirmed that the system significantly promoted the proliferation, osteogenic differentiation, and mineralization of bone marrow mesenchymal stem cells (BMSCs), while upregulating key osteogenic markers such as RUNX2, ALP, BSP, and Col1. Importantly, in a rat critical-sized calvarial defect model, the composite scaffold group exhibited markedly increased bone formation (BV/TV) and improved bone quality at 8 weeks compared to single-component controls. In summary, this integrated strategy, combining "hematoma stabilization" with "osteogenic induction", effectively meets the sequential demands of early hemostasis and subsequent bone regeneration, presenting a promising strategy for the development of advanced bone repair materials.