文献类型：期刊文献

英文题名：The extracts of small-leaved kudingcha as potential modulators of β-cells for the therapy of type 2 diabetes mellitus

作者：Chen, MingJuan Huang, Lirong Xiong, Qinqin Zan, Ningning Wang, Zhi Bai, Yongfen Yu, Jia Cheng, Sha Zeng, Hong Ahmad, Mashaal Luo, Heng

第一作者：Chen, MingJuan

通信作者：Luo, H[1]

机构：[1]Guizhou Med Univ, State Key Lab Funct & Applicat Med Plants, Guiyang 550014, Peoples R China;[2]Nat Prod Res Ctr Guizhou Prov, Guiyang 550014, Peoples R China;[3]Guizhou Inst Technol, Coll Food & Pharmaceut Engn, Guiyang 550025, Peoples R China;[4]Tea Ind Dev Serv Ctr Yuqing Cty, Zunyi 564400, Peoples R China

第一机构：Guizhou Med Univ, State Key Lab Funct & Applicat Med Plants, Guiyang 550014, Peoples R China

通信机构：corresponding author), Guizhou Med Univ, State Key Lab Funct & Applicat Med Plants, Guiyang 550014, Peoples R China.

年份：2025

卷号：130

外文期刊名：JOURNAL OF FUNCTIONAL FOODS

收录：;Scopus(收录号：2-s2.0-105006944087);WOS:【SCI-EXPANDED(收录号:WOS:001503624100001)】；

基金：This work is supported by Guizhou Province "100" level Innovative Talents Training Project in the Guizhou Provincial Committee Organization Department (no. QKHPTRCGCC [2022] 034-1) , Guizhou Institute Technology high-level talent research start-up funding project (XJGC20190659) and Zunyi City science and technology innovation talent team training program (no. ZSKRC [2023] 10) .

语种：英文

外文关键词：Small-leaved Kudingcha (SLK); Type 2 diabetes mellitus (T2DM); Islet (3-cells; PI3K/AKT; Safety evaluation

摘要：Small-leaved Kudingcha (SLK), a unique variety of tea, has blood sugar-lowering effect. However, the underlying molecular mechanisms of lowering blood sugar is still unclear. Here, the crude extract of SLK and its four fractions of petroleum ether (SLK-PE), ethyl acetate (SLK-EA), n-butanol (SLK-NA), and water (SLK-WA) were prepared and analyzed by Liquid Chromatography-Mass spectrometry/Mass spectrometry. The findings revealed that SLK-PE and SLK-NA ameliorated polydipsia, polyphagia, and polyuria in T2DM mice, as well as serum biochemical indicators. We found that SLK-PE and SLK-NA could enhance blood sugar regulation ability and improve the degree of islet cells damage by upregulating the relative protein levels of p-PI3K/PI3K and p-AKT/ AKT ratios, as well as Glut4, Nrf2, INS, SIRT1 and PGC-1a, while downregulating the expression levels of Keap-1, FoXO1, GSK3(3, and GCG. Cellular experiment results further demonstrated that SLK-PE and SLK-NA could promote MIN6 cells proliferation and increasing insulin secretion via PI3K/AKT signaling pathway. Additionally, SLK-PE and SLK-NA have a high level of safety. The findings demonstrate that SLK-PE and SLK-NA could serve as potential functional additives for the prophylaxis and management of diabetes and linked metabolic conditions.