文献类型：期刊文献

英文题名：An amphiphilic fluorescent polymer combining aggregation-induced emission monomer and epsilon-polylysine for cell imaging

作者：Xie, Gaoyi Ma, Chunping Zhang, Xiqi Liu, Hongliang Gong, Jialiang Zhao, Shuyang Li, Yang Wang, Ke Wei, Yen

第一作者：谢高艺

通信作者：Ma, CP[1];Zhang, XQ[2];Wei, Y[3];Wei, Y[4]|[144404c59386af0196f94]马春平;

机构：[1]Guizhou Inst Technol, Coll Chem Engn, Guiyang 550003, Guizhou, Peoples R China;[2]Guizhou Inst Technol, Coll Mat & Met Engn, Guiyang 550003, Guizhou, Peoples R China;[3]Chinese Acad Sci, Tech Inst Phys & Chem, CAS Key Lab Bioinspired Mat & Interface Sci, Beijing 100190, Peoples R China;[4]Tsinghua Univ, Dept Chem, Beijing 100084, Peoples R China;[5]Tsinghua Univ, Tsinghua Ctr Frontier Polymer Res, Beijing 100084, Peoples R China;[6]Sun Yat Sen Univ, Sch Pharmaceut Sci, Guangzhou 510006, Guangdong, Peoples R China

第一机构：贵州理工学院化学工程学院

通信机构：corresponding author), Guizhou Inst Technol, Coll Mat & Met Engn, Guiyang 550003, Guizhou, Peoples R China;corresponding author), Chinese Acad Sci, Tech Inst Phys & Chem, CAS Key Lab Bioinspired Mat & Interface Sci, Beijing 100190, Peoples R China;corresponding author), Tsinghua Univ, Dept Chem, Beijing 100084, Peoples R China;corresponding author), Tsinghua Univ, Tsinghua Ctr Frontier Polymer Res, Beijing 100084, Peoples R China.|贵州理工学院材料与冶金工程学院;贵州理工学院;

年份：2017

卷号：145

起止页码：174-180

外文期刊名：DYES AND PIGMENTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000405972900022)】；

基金：This research was supported by the National Science Foundation of China (Nos. 51603211, 51503046, 51673107, 21604048), the MOST of China (2016YFA200800), the Guizhou Province Science and Technology Planning Project ([2015]2068), and the Scientific&Technological Innovation Talent Team of Guizhou Province (Qian Ke He Talent Team [2015]4008).

语种：英文

外文关键词：Aggregation-induced emission; epsilon-Polylysine; Cross-linked fluorescent polymer; Critical micelle concentration; Photostability; Biocompatibility

摘要：An amphiphilic fluorescent polymer (Ply-EP3) combining an aggregation-induced emission monomer and e-polylysine was prepared through epoxy ring-opening polymerization. H-1 NMR, FT-IR, and X-ray photoelectron spectra were conducted to characterize its chemical property. Such amphiphilic Ply-EP3 could assemble into nanoparticles in water and form fluorescent polymeric nanoparticles (FPNs) with high water dispersibility. Dynamic light scattering result of PIy-EP3 FPNs demonstrated the size distribution of 106 +/- 8 nm with the polydispersity index of 0.268. Transmission electron microscopy image of Ply-EP3 FPNs showed spherical nanoparticle morphology with a size range of 50-100 nm. The optical property of FPNs indicated intense green fluorescence with a fluorescence quantum yield-of 33%. The PlyEP3 FPNs further proved excellent stability with low critical micelle concentration of 0.042 mg mL(-1). The prepared FPNs also revealed high photostability after UV light irradiation for 120 min. Such FPNs showed excellent biocompatibility as the cells retained high viability at the concentration range of 10 -120 mu g mL(-1) of Ply-EP3 for an incubated period of 12 h or 24 h, and exhibited excellent performance for cell imaging. Thanks to the high-performance Ply-EP3 FPNs, including bright fluorescent emission, high water dispersibility and particle stability, and excellent photostability, this work provide an approach to prepare robust cross-linked biocompatible fluorescent polymers for cell imaging. (C) 2017 Elsevier Ltd. All rights reserved.