文献类型：期刊文献

英文题名：Oxidized epigallocatechin gallate inhibited lysozyme fibrillation more strongly than the native form

作者：An, Ting-Ting Feng, Shuang Zeng, Cheng-Ming

第一作者：An, Ting-Ting

通信作者：Zeng, CM[1]

机构：[1]Shaanxi Normal Univ, Sch Chem & Chem Engn, Key Lab Analyt Chem Life Sci Shaanxi Prov, Xian 710119, Peoples R China;[2]Guizhou Inst Technol, Sch Chem Engn, Guiyang 550003, Peoples R China

第一机构：Shaanxi Normal Univ, Sch Chem & Chem Engn, Key Lab Analyt Chem Life Sci Shaanxi Prov, Xian 710119, Peoples R China

通信机构：corresponding author), Shaanxi Normal Univ, Sch Chem & Chem Engn, Key Lab Analyt Chem Life Sci Shaanxi Prov, Xian 710119, Peoples R China.

年份：2017

卷号：11

起止页码：315-321

外文期刊名：REDOX BIOLOGY

收录：;Scopus(收录号：2-s2.0-85007035090);WOS:【SCI-EXPANDED(收录号:WOS:000398212000032)】；

语种：英文

外文关键词：Epigallocatechin gallate; Oxidation; Anti-amyloidogenic activity; Lysozyme; Amyloid fibrils; Cytotoxicity

摘要：Epigallocatechin gallate (EGCG), the most abundant flavanoid in green tea, is currently being evaluated in the clinic due to its benefits in the treatment of amyloid disorders. Its anti-amyloidogenic effect has been attributed to direct interaction of the intact molecule with misfolded polypeptides. In addition, antioxidant activity is also involved in the anti-amyloidogenic role. The detailed molecular mechanism is still unclear and requires further investigation. In the present study, the kinetics of EGCG oxidation and the anti-amyloidogenic effect of the resultant oxidation substances have been examined. The results indicate that EGCG degrades in a medium at pH 8.0 with a half-life less than 2 h. By utilizing lysozyme as an in vitro model, the oxidized EGCG demonstrates a more potent anti-amyloidogenic capacity than the intact molecule, as shown by ThT and ANS fluorescence, TEM determination, and hemolytic assay. The oxidized EGCG also has a stronger disruptive effect on preformed fibrils than the native form. Ascorbic acid eliminates the disruptive role of native EGCG on the fibrils, suggesting that oxidation is a prerequisite in fibril disruption. The results of this work demonstrate that oxidized EGCG plays a more important role than the intact molecule in anti-amyloidogenic activity. These insights into the action of EGCG may provide a novel route to understand the anti-amyloidogenic activity of natural polyphenols.