文献类型：期刊文献

英文题名：Diversity and importance of culturable endophytic fungi of tobacco (Nicotinana tabacum) cultivated in southwestern China

作者：Wang, Maosheng Sun, Jialong Wei, Yali Jia, Yanlong Zou, Xiao

第一作者：Wang, Maosheng

通信作者：Sun, JL[1]

机构：[1]Guizhou Inst Technol, Sch Food & Pharmaceut Engn, Guiyang, Peoples R China;[2]Guizhou Acad Tobacco Sci, Key Lab Mol Genet, Guiyang, Peoples R China;[3]Guizhou Inst Technol, Sch Resources & Environm Engn, Guiyang 550001, Peoples R China;[4]Guizhou Minzu Univ, Sch Ethn Minor Med, Guiyang, Peoples R China;[5]Guizhou Univ, Inst Fungal Resources, Guiyang, Peoples R China

第一机构：贵州理工学院食品药品制造工程学院

通信机构：corresponding author), Guizhou Inst Technol, Sch Resources & Environm Engn, Guiyang 550001, Peoples R China.|贵州理工学院资源与环境工程学院;贵州理工学院;

年份：0

外文期刊名：JOURNAL OF PHYTOPATHOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000815358300001)】；

基金：This work was supported by Major Program for Innovative Research Groups from Guizhou Provincial Department of Education (Qian-KY[2016]045) and Research Foundation for Advanced Scholars of Guizhou Institute of Technology (XJGC--20190638).

语种：英文

外文关键词：antagonistic effect; endophytic fungi; fungal diversity; Nicotiana tabacum; nicotianae; Phytophthora parasitica var

摘要：Tobacco (Nicotiana tabacum) is one of the most important economic crops in China; however, black shank caused by Phytophthora parasitica is major threat to its cultivation. So far, little is known regarding potential antagonistic effects of endophytes on plant pathogens. A total of 109 culturable endophytic fungal strains were recovered from various tissues of six tobacco cultivars that are commonly cultivated in southwestern China. Morphological and molecular identification showed that these isolates belonged to 18 different genera, including Aspergillus, Chaetomium, Gibellulopsis and Penicillium as predominant genera, which were frequently observed in stem, leaf and root tissues. Among them, only 17 endophytic fungal strains exerted antagonistic effects on P. parasitica in the dual-culture test, and the highest inhibition rate of 73% was produced by a Penicillium janthinellum strain isolated from stem tissue of a cultivar HD. Nevertheless, a field trial revealed that Shannon-Wiener indices were negatively correlated with the disease index and with the incidence of tobacco blank shank in cultivars K326, YUN85, D101 and GUI1, which was further supported by the evaluation of endophytic community similarity. Our results contribute to the understanding of functions and diversity of endophytic fungi, and culturable endophytic fungal strains may be considered potential libraries for screening novel bioactive and effective metabolites to control P. parasitica in crops which requires further research.