文献类型：期刊文献

英文题名：Ethylicin Inhibition of Xanthomonas oryzae pv. oryzicola In Vitro and In Vivo

作者：Huang, Yajiao Li, Hongde Zhao, Guili Bai, Qian Huang, Min Luo, Dan Li, Xiangyang

第一作者：Huang, Yajiao

通信作者：Li, XY[1]

机构：[1]Guizhou Univ, Minist Educ, State Key Lab Breeding Base Green Pesticide & Agr, Key Lab Green Pesticide & Agr Bioengn, Guiyang 550025, Peoples R China;[2]Guizhou Inst Technol, Coll Chem Engn, Guiyang 550003, Peoples R China

第一机构：Guizhou Univ, Minist Educ, State Key Lab Breeding Base Green Pesticide & Agr, Key Lab Green Pesticide & Agr Bioengn, Guiyang 550025, Peoples R China

通信机构：corresponding author), Guizhou Univ, Minist Educ, State Key Lab Breeding Base Green Pesticide & Agr, Key Lab Green Pesticide & Agr Bioengn, Guiyang 550025, Peoples R China.

年份：2023

卷号：71

期号：3

起止页码：1405-1416

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20230413426609);Scopus(收录号：2-s2.0-85146555071);WOS:【SCI-EXPANDED(收录号:WOS:000929008800001)】；

基金：This work was supported in part by the National Key Research and Development Program of China (Grant number 2021YFD1700101) and National Natural Science Foundation of China (31960546 and 32172461), Talents of Guizhou Science and Technology Cooperation Platform [(2021)5623], Guizhou Science and Technology Cooperation Foundation [ZK(2021)140], the Unique Feature Project of Guizhou Provincial Education Department [KY(2021)056], and the Program of Introducing Talents of Discipline to Universities of China (111 Program, D20023).

语种：英文

外文关键词：bacterial leaf streak; Xanthomonas oryzae pv. oryzicola; ethylicin; antibacterial action; proteomics; qRT-PCR

摘要：Infestation of rice with the bacterium Xanthomonas oryzae pv. oryzicola (Xoc) causes the serious disease bacterial leaf streak (BLS). We studied the effect of ethylicin, a broad-spectrum bactericide, on Xoc both in vivo and in vitro. Ethylicin increases the defensive enzyme activities and defensive genes expression of rice. Ethylicin also significantly inhibited Xoc activity in vitro compared with other commercial bactericides. The half-maximal effective concentration (EC50) of ethylicin was 2.12 mu g/mL. It has been shown that ethylicin can inhibit Xoc quorum sensing through the production of extracellular polysaccharides and enzymes, which disrupt the Xoc cell membrane. We used proteomic analysis to identify two oxidative phosphorylation pathway proteins (ACU12_RS13405 and ACU12_RS13355) which affected the virulence of Xoc and validated them using quantitative real-time polymerase chain reaction (qRT-PCR). The results indicate that ethylicin can increase the defense responses of rice and control Xoc proliferation.