文献类型：期刊文献

英文题名：Engineering of a chitosanase fused to a carbohydrate-binding module for continuous production of desirable chitooligosaccharides

作者：Zhou, Jianli Harindintwali, Jean Damascene Yang, Wenhua Han, Minghai Deng, Bin Luan, Hao Zhang, Wenshuai Liu, Xiaobo Yu, Xiaobin

第一作者：Zhou, Jianli

通信作者：Liu, XB[1];Yu, XB[1]

机构：[1]Jiangnan Univ, Key Lab Carbohydrate Chem & Biotechnol, Minist Educ, 1800 Lihu Rd, Wuxi 214122, Jiangsu, Peoples R China;[2]Guizhou Inst Technol, Sch Food & Pharmaceut Engn, 1 Caiguan Rd, Guiyang 550003, Peoples R China;[3]Guangdong Tech Israel Inst Technol, Environm Engn Program, 241 Daxue Rd, Shantou 515063, Peoples R China

第一机构：Jiangnan Univ, Key Lab Carbohydrate Chem & Biotechnol, Minist Educ, 1800 Lihu Rd, Wuxi 214122, Jiangsu, Peoples R China

通信机构：corresponding author), Jiangnan Univ, Sch Biotechnol, 1800 Lihu Rd, Wuxi 214122, Jiangsu, Peoples R China.

年份：2021

卷号：273

外文期刊名：CARBOHYDRATE POLYMERS

收录：;EI(收录号：20213610854212);Scopus(收录号：2-s2.0-85114002415);WOS:【SCI-EXPANDED(收录号:WOS:000702870300005)】；

基金：This project was financially supported by the National First-class Discipline Program of the Light Industry Technology and Engineering (Grant No. LITE2018-11), the Open Project of Key Laboratory of Carbohydrate Chemistry and Biotechnology Ministry of Education (Grant No. KLCCB-KF202007), the Guizhou Science and Technology Plan Project (Grant No. [2020]1Y144), and the Natural Science Research Project of Education Department of Guizhou Province (Grant No. KY [2015]368).

语种：英文

外文关键词：Bioproduction; Chitooligosaccharide; Chitosanase; Carbohydrate-binding module; One-step purification and immobilization; Packed-bed reactor

摘要：Chitooligosaccharides (CHOS) with multiple biological activities are usually produced through enzymatic hydrolysis of chitosan or chitin. However, purification and recycling of the enzyme have largely limited the advancement of CHOS bioproduction. Here, we engineered a novel enzyme by fusing the native chitosanase Csn75 with a carbohydrate-binding module (CBM) that can specifically bind to curdlan. The recombinase Csn75CBM was successfully expressed by Pichia pastoris and allowed one-step purification and immobilization in the chitosanase immobilized curdlan packed-bed reactor (CICPR), where a maximum adsorption capacity of 39.59 mg enzyme/g curdlan was achieved. CHOS with degrees of polymerization of 2-5 (a hydrolysis yield of 97.75%), 3-6 (75.45%), and 3-7 (73.2%) were continuously produced by adjusting the ratio of enzyme and chitosan or the flow rate of chitosan. Moreover, the CICPR exhibited good stability and reusability after several cycles. The recombinase Csn75-CBM has greatly improved the efficiency of the bioproduction of CHOS.