文献类型：期刊文献

英文题名：Triplex DNA logic gate based upon switching on/off their structure by Ag+/cysteine

作者：Xiao, Zhiyou Zhu, Houya Xin, Aiping Li, Yubin Ling, Liansheng

第一作者：Xiao, Zhiyou;肖志友

通信作者：Ling, LS[1]

机构：[1]Sun Yat Sen Univ, Sch Chem & Chem Engn, Guangzhou 510275, Guangdong, Peoples R China;[2]Guizhou Inst Technol, Sch Chem Engn, Guiyang 550003, Peoples R China;[3]Guangdong Med Instruments Qual Surveillance & Ins, Packaging Mat & Containers Test Ctr, Guangzhou 50663, Guangdong, Peoples R China

第一机构：Sun Yat Sen Univ, Sch Chem & Chem Engn, Guangzhou 510275, Guangdong, Peoples R China

通信机构：corresponding author), Sun Yat Sen Univ, Sch Chem & Chem Engn, Guangzhou 510275, Guangdong, Peoples R China.

年份：2015

卷号：140

期号：21

起止页码：7322-7326

外文期刊名：ANALYST

收录：;Scopus(收录号：2-s2.0-84944112057);WOS:【SCI-EXPANDED(收录号:WOS:000362795100030)】；

基金：The study was supported by the National Natural Science Foundation of China (no. 21375153), the Fundamental Research Funds for the Central Universities (no. 13lgzd05), the open project of Beijing National Laboratory for Molecular Sciences and the Science and Technology Foundation of Guizhou Province (Qian Ke He J[2013]NO.2263).

语种：英文

摘要：The formation of intramolecular triplex DNA can be regulated by Ag+ and Cys (cysteine), which switch off/on the fluorescence of the oligonucleotides, 5'-TAMRA-TTC TCT TCC TCT TCC TTC TGA CGA CAG TTG ACT CTT CCT TCT CCT TCT CTT-BHQ-2-3' (Oligo 1) and 3'-GAA GGA AGA GGA AGA GAA-5' (Oligo 2). Based on this principle, sensors for Ag+ and Cys are developed. The sensor for Ag+ has a linear range of 2.5 nM-40 nM and a detection limit of 1.8 nM, whereas the sensor for Cys has a linear range of 10.0 nM-120.0 nM and a detection limit of 8.2 nM. Furthermore, the fluorescence is reversible with the alternate addition of Ag+ and Cys. We constructed a DNA logic gate using Ag+ and Cys as the input, and the fluorescence intensity as the output. The DNA logic gate is simple; moreover, it has a fast response and good reversibility.