文献类型：期刊文献

英文题名：Hypotensive effect of potent angiotensin-I-converting enzyme inhibitory peptides from corn gluten meal hydrolysate: Gastrointestinal digestion and transepithelial transportation modifications

作者：Chanajon, Phiromya Hamzeh, Ali Tian, Fu Roytrakul, Sittiruk Oluwagunwa, Olayinka A. Kadam, Deepak Aluko, Rotimi E. Aueviriyavit, Sasitorn Wongwanakul, Ratjika Yongsawatdigul, Jirawat

第一作者：Chanajon, Phiromya

通信作者：Yongsawatdigul, J[1];Aluko, RE[2]

机构：[1]Suranaree Univ Technol, Sch Food Technol, Inst Agr Technol, Nakhon Ratchasima 30000, Thailand;[2]Guizhou Inst Technol, Coll Food & Pharmaceut Engn, Guiyang 550000, Peoples R China;[3]Natl Ctr Genet Engn & Biotechnol, Pathum Thani 12120, Thailand;[4]Univ Manitoba, Dept Food & Human Nutr Sci, Winnipeg, MB R3T 2N2, Canada;[5]Natl Sci & Technol Dev Agcy, Natl Nanotechnol Ctr, 111 Thailand Sci Pk, Pathum Thani 12120, Thailand

第一机构：Suranaree Univ Technol, Sch Food Technol, Inst Agr Technol, Nakhon Ratchasima 30000, Thailand

通信机构：corresponding author), Suranaree Univ Technol, Sch Food Technol, Inst Agr Technol, Nakhon Ratchasima 30000, Thailand;corresponding author), Univ Manitoba, Dept Food & Human Nutr Sci, Winnipeg, MB R3T 2N2, Canada.

年份：2025

卷号：462

外文期刊名：FOOD CHEMISTRY

收录：;EI(收录号：20243516960036);Scopus(收录号：2-s2.0-85202344017);WOS:【SCI-EXPANDED(收录号:WOS:001316460500001)】；

基金：PC received a scholarship from the Royal Golden Jubilee Ph.D. Program of Thailand (grant number PhD/0060/2558) . Research funding was supported by the following: (1) Suranaree University of Technology (SUT) , (2) Thailand Science Research and Innovation (TSRI) , and (3) the National Science, Research and Innovation Fund (NSRF) under the Fundamental Fund Project (NRIIS number 179296) . Additional funding, which came from the National Research Council of Thailand (N42A650548) and the Office of the Permanent Secretary of the Ministry of Higher Education, Science, Research and Innovation were appreciated.

语种：英文

外文关键词：Angiotensin I-converting enzyme; Caco-2 cells; Molecular docking; Molecular dynamics; Spontaneously hypertensive rats; Corn gluten meal

摘要：The study examined the antihypertensive effect of peptides derived from pepsin-hydrolyzed corn gluten meal, namely KQLLGY and PPYPW, and their in silico gastrointestinal tract digested fragments, KQL and PPY, respectively. KQLLGY and PPYPW showed higher angiotensin I-converting enzyme (ACE)-inhibitory activity and lower ACE inhibition constant (K-i) values when compared to KQL and PPY. Only KQL showed a mild antihypertensive effect in spontaneously hypertensive rats with -7.83 and - 5.71 mmHg systolic and diastolic blood pressure values, respectively, after 8 h oral administration. During passage through Caco-2 cells, KQL was further degraded to QL, which had reduced ACE inhibitory activity. In addition, molecular dynamics revealed that the QL-ACE complex was less stable compared to the KQL-ACE. This study reveals that structural transformation during peptide permeation plays a vital role in attenuating antihypertensive effect of the ACE inhibitor peptide.