文献类型：期刊文献

英文题名：Using a Proteomic Approach to Differentiate Saccharomyces cerevisiae for Superior Alternative Protein Sources

作者：Zhao, Yan Chen, Bingyu Zhang, Xiaoyue Zhu, Xuchun Huang, Mingzheng Liu, Hongzhi

第一作者：Zhao, Yan

通信作者：Liu, HZ[1];Huang, MZ[2]

机构：[1]Beijing Technol & Business Univ, Sch Food & Hlth, Beijing 100080, Peoples R China;[2]Guizhou Inst Technol, Sch Food & Pharmaceut Engn, Guiyang 550003, Guizhou, Peoples R China

第一机构：Beijing Technol & Business Univ, Sch Food & Hlth, Beijing 100080, Peoples R China

通信机构：corresponding author), Beijing Technol & Business Univ, Sch Food & Hlth, Beijing 100080, Peoples R China;corresponding author), Guizhou Inst Technol, Sch Food & Pharmaceut Engn, Guiyang 550003, Guizhou, Peoples R China.|贵州理工学院食品药品制造工程学院;贵州理工学院;

年份：2025

卷号：73

期号：39

起止页码：25065-25075

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20254019267948);Scopus(收录号：2-s2.0-105017494859);WOS:【SCI-EXPANDED(收录号:WOS:001577791700001)】；

基金：The authors thank the following organizations for supporting this study: The National Natural Science Foundation of China (ID: 32472269), Guizhou Provincial Science and Technology Department (KXJZ[2024]021), and Beijing High-level Talent Project (ID: 19008024075).

语种：英文

外文关键词：Saccharomyces cerevisiae; proteomic; cell proliferation; alternative protein

摘要：This study aims to screen yeast strains with a high protein content from a proteomics perspective, with the aim of exploring potential sustainable sources of alternative protein. A comparative proteomics analysis of four nonindustrial brewing yeasts revealed that proteins involved in synthesis and degradation were significantly more prevalent in high-protein strains. Yeast-A (Y-A) strain, which had the highest protein content (54.26 +/- 0.89/100 g) and fastest growth rate, was selected for in-depth functional analysis. Differentially expressed proteins (such as PAI3 and PEP4) were identified using fold change (FC > 2 or FC < 1/2) and p-value (p < 0.01) as thresholds. These findings imply that protein synthesis and degradation pathways coordinate transcription and translation to regulate protein content and growth. Future research should focus on optimizing these pathways through genetic engineering to enhance the efficiency with which yeast produces protein.