文献类型：期刊文献

中文题名：贵州何首乌HPLC指纹图谱研究

英文题名：HPLC Fingerprint Chromatogram of Polygonum multiflorum from Guizhou

作者：李燕 王慧娟 林冰 赵致 周英

第一作者：李燕

机构：[1]贵州理工学院;[2]贵州大学;[3]贵州大学中药天然药研发中心;[4]贵州省中药材繁育与种植重点(工程)实验室

第一机构：贵州理工学院

年份：2012

卷号：35

期号：12

起止页码：1928-1932

中文期刊名：中药材

外文期刊名：Journal of Chinese Medicinal Materials

收录：MEDLINE(收录号：23705355);CSTPCD;;Scopus(收录号：2-s2.0-84955367810);北大核心:【北大核心2011】；CSCD:【CSCD2011_2012】；PubMed;

基金：国家科技支撑计划子课([2009]BA174B01-2-12);贵州省优秀科技教育人才省长专项资金项目(黔省专合字2010)

语种：中文

中文关键词：何首乌;指纹图谱;高效液相色谱

外文关键词：Polygonum multiflorum Thunb.; Fingerprint chromatogram; HPLC

摘要：目的:建立贵州产何首乌药材的指纹图谱,为其质量控制提供依据。方法:采用高效液相色谱法,色谱柱:Agillent ZABAX-C18(4.6 mm×250 mm,5μm),流动相:乙腈-0.4%磷酸水溶液梯度洗脱,流速:1.0 mL/min,柱温:25℃,检测波长:280 nm,分析时间:60 min。结果:对贵州不同产地的药材样品进行分析,确定了9个共有峰,精密度、稳定性、重复性试验中各共有峰相对保留时间和相对峰面积的RSD均小于3%,16批样品相似度均达0.9以上。结论:所建立的方法简便、可靠,可为何首乌药材的质量控制提供指导和依据。
Objective：To establish the fingerprint of Polygonum multiflorum from Guizhou and provide a standard for its quality control.Methods：HPLC analysis was performed on Agillent ZABAX-C18（4.6 mm×250 mm,5μm）,gradient eluted composed of acetonitrile-0.4% water solution of phosphoric acid.Column temperature was set at 25 ℃and the flow rate was 1 mL/min.The detection wavelength was 280 nm and the analysis time was 60 min.Results：9 common peaks were identified.The RSD of the relative retention time and the relative peak area were less than 3% in analyzing its precision,stability and repeatability of the common peaks,and the similarity of the 16 batches of sample was more than 0.9.Conclusion：The method is simple and reliable,and it can provide a standard and guidance for quality control of Polygonum multiflorum.