文献类型：期刊文献

中文题名：单因素实验结合响应面法优化朱砂根总皂苷的提取工艺

英文题名：Optimization of Extraction Technology for Total Saponins of Ardisia Crenata by Singlefactor Experiment Combined with Response Surface Methodology

作者：吴琴 陈珣

第一作者：吴琴

机构：[1]贵州理工学院制药工程学院;[2]毕节学院化学工程学院

第一机构：贵州理工学院食品药品制造工程学院

年份：2014

卷号：33

期号：8

起止页码：1080-1082

中文期刊名：医药导报

外文期刊名：Herald of Medicine

收录：CSTPCD;;北大核心:【北大核心2011】；

语种：中文

中文关键词：朱砂根;总皂苷;响应面法;提取工艺

外文关键词：Ardisia crenata;Total saponins;Response surface methodology;Optimization of extraction

摘要：目的优化朱砂根的最佳提取工艺,为朱砂根的研究提供基础。方法以朱砂根总皂苷的含量为指标,在单因素实验的基础上,根据Box-Behnken中心组合实验设计原理,设计3因素3水平的响应面法实验,优化朱砂根的最佳提取工艺。结果单因素实验结合响应面法优化得到的朱砂根最佳提取工艺为:以70%乙醇为溶剂,提取温度70℃,料液比为1∶16,提取时间为3 h,在此条件下,总皂苷百分含量的理论预测值为2.29%。结论通过响应面法优化得到的朱砂根总皂苷提取工艺稳定、可靠,具有一定的实用价值。
Objective To optimize the extraction process of Ardisia crenata based on the content of total saponins and to provide a basis for the Ardisia crenata study. Methods Based on the results of the single-factor tests and the Box-Behnken central composite experimental design principles, a response surface methodology which has three factors and three levels was designed to optimize the extraction process of Ardisia crenata based on the content of saponins. Results A maximal extraction yield of total saponins reached 2. 29% under the optimal conditions as follows：70% alcohol was used as extraction solvent with the material to liquid ratio of 116, the extraction time was 3 h at 70 ℃. Conclusion The optimized extraction process is accurate, reliable and practically valuable.