文献类型：期刊文献

英文题名：Piperidine-functionalized formononetin derivatives: Design, synthesis and evaluation of antibacterial activity, modes and phenotypes

作者：Zhang, Miaohe Song, Junrong Wei, Xian Hao, Gefei Jin, Linhong Jayawardana, Barana C. Marambe, Buddhi Feng, Shuang Xue, Wei

第一作者：张妙鹤

通信作者：Feng, S[1];Feng, S[2];Xue, W[2]

机构：[1]Guizhou Inst Technol, Sch Chem Engn, Guiyang 550025, Peoples R China;[2]Guizhou Univ, Ctr R&D Fine Chem, Guiyang 550025, Peoples R China;[3]Guizhou Med Univ, Nat Prod Res Ctr Guizhou Prov, State Key Lab Discovery & Utilizat Funct Component, Guiyang, Peoples R China;[4]Univ Peradeniya, Fac Agr, Dept Anim Sci, Peradeniya, Sri Lanka;[5]Univ Peradeniya, Fac Agr, Dept Crop Sci, Peradeniya, Sri Lanka

第一机构：贵州理工学院化学工程学院

通信机构：corresponding author), Guizhou Inst Technol, Sch Chem Engn, Guiyang 550025, Peoples R China;corresponding author), Guizhou Univ, Ctr R&D Fine Chem, Guiyang 550025, Peoples R China.|贵州理工学院化学工程学院;贵州理工学院;

年份：2026

外文期刊名：PEST MANAGEMENT SCIENCE

收录：;Scopus(收录号：2-s2.0-105042127416);WOS:【SCI-EXPANDED(收录号:WOS:001794525400001)】；

基金：The authors gratefully acknowledge the National Key Research and Development Program of China (no. 2024YFE0214300), and the Natural Science Foundation of Guizhou Province (grant nos QKHJC-MS [2026]240 and QKHJC-MS [2026]388).

语种：英文

外文关键词：antibacterial activity; antibacterial modes; antibacterial phenotypes; formononetin derivatives; piperidine functionalization

摘要：BACKGROUND Plant pathogenic bacteria severely impact crop yield and quality. Long-term use of conventional bactericides has led to resistance, and raised environmental and food safety concerns, necessitating new, efficient and eco-friendly agents.RESULTS This study designed and synthesized a series of novel piperidine-functionalized formononetin derivatives (N1-N42) and evaluated their antibacterial activity. In vitro assays showed that several compounds strongly inhibited Xanthomonas oryzae pv. oryzae (Xoo) and Xanthomonas axonopodis pv. citri (Xac). Notably, thiourea-piperidine N30 and piperidine hydrochloride N42 were highly effective against Xoo, with median effective concentration (EC50) values of 6.5 and 4.0 mu g mL-1, respectively, outperforming the controls thiodiazole copper (TC, 33.3 mu g mL-1) and zinc thiazole (ZT, 13.9 mu g mL-1). N42 also exhibited potent activity against Xac (EC50 = 7.5 mu g mL-1), superior to activities of TC (30.7 mu g mL-1) and ZT (58.7 mu g mL-1). In vivo pot experiments confirmed that N30 exhibited good efficacy against rice bacterial leaf blight (BLB), whereas N42 showed dual efficacy in controlling both BLB and citrus canker (CC). Further investigation of the antibacterial modes and related phenotypes revealed that N30 and N42 could cause bacterial cell-envelope damage, decrease biofilm formation and exopolysaccharide (EPS) production, attenuate swarming motility, reduce the activities of extracellular virulence-related enzymes, and enhance the activities of defense-related enzymes in host plants.CONCLUSION N30 and N42 have good potential to be developed as formononetin-based antibacterial agents.