文献类型：期刊文献

英文题名：Rapid Colorimetric Determination of microRNA (miRNA) Using an Autocatalytic Entropy-Driven Circuit (AEDC)

作者：Dai, Jianyuan Dong, Guixiu Mao, Jianfei Liu, Yunhua Lin, Fengyi Cheng, Yuxin Yi, Huaichao Zhou, Cuisong Guo, Yong Xiao, Dan

第一作者：Dai, Jianyuan

通信作者：Dai, JY[1]

机构：[1]Sichuan Univ, Coll Chem, Chengdu, Peoples R China;[2]Xinyun Elect Com & Dev Co Ltd, China Zhenhua Grp, Guiyang, Peoples R China;[3]Guizhou Inst Technol, Sch Chem Engn, Guiyang, Peoples R China;[4]Sichuan Univ, Coll Chem Engn, Chengdu, Sichuan, Peoples R China;[5]Sichuan Univ, Coll Chem, Chengdu 610064, Peoples R China

第一机构：Sichuan Univ, Coll Chem, Chengdu, Peoples R China

通信机构：corresponding author), Sichuan Univ, Coll Chem, Chengdu 610064, Peoples R China.

年份：2023

外文期刊名：ANALYTICAL LETTERS

收录：;Scopus(收录号：2-s2.0-85180491615);WOS:【SCI-EXPANDED(收录号:WOS:001129834000001)】；

基金：No Statement Available

语种：英文

外文关键词：Autocatalytic entropy-driven circuit (AEDC); colorimetry; microRNA (miRNA); serum analysis; spectrophotometry

摘要：A colorimetric and rapid signal amplified microRNA (miRNA) detection system based on autocatalytic entropy-driven circuit (AEDC) is reported. The target miRNA-21 (miR-21) was firstly hybridized with the sticky end of the triple-stranded hybrid complex (S, which consisting of a reporter strand (P), a byproduct strand (R) and an assistant strand (Q)), and P strand, which contained the G-quadruplex sequence that was released through the toehold-mediated strand displacement reaction. The G-quadruplex sequence interacted with hemin to form the peroxidase-mimicking DNAzymes and catalyzed the H2O2-mediated oxidation of colorless 2,2 '-azinobis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS2-) to the green ABTS center dot-. Meanwhile, fuel strand (F) hybridized with S to release the target miR-21 and the waste duplex DNA product F-Q was formed, which brings the two split mimic target DNA sequences into proximity to form the intact mimic target DNA sequence (analogue of miR-21). The obtained mimic target DNA as well as the released miR-21 can be cyclically used to trigger autocatalytic EDC reaction, leading to the rapidly enhanced formation of mimic target DNA with the concomitant generation of a higher colorimetric signal. Finally, a colorimetric and rapid miR-21 system based on the hairpin-free AEDC reaction was successfully fabricated. This system also has been applied for the miRNA determination in serum, exhibiting the potential application in the clinical diagnosis.