文献类型：期刊文献

英文题名：Isolation and Identification of an alpha-Galactosidase-Producing Lactosphaera pasteurii Strain and Its Enzymatic Expression Analysis

作者：Zhao, Yan Zhou, Jinghui Dai, Shan Liu, Xiaozhu Zhang, Xuewen

第一作者：Zhao, Yan

通信作者：Zhang, XW[1];Zhang, XW[2];Liu, XZ[3]

机构：[1]Key Lab Crop Epigenet Regulat & Dev Hunan Prov, Changsha 410128, Peoples R China;[2]Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China;[3]Hunan Agr Univ, Coll Hort, Changsha 410128, Peoples R China;[4]Guizhou Inst Technol, Coll Food & Pharmaceut Engn, Guiyang 550000, Peoples R China

第一机构：Key Lab Crop Epigenet Regulat & Dev Hunan Prov, Changsha 410128, Peoples R China

通信机构：corresponding author), Key Lab Crop Epigenet Regulat & Dev Hunan Prov, Changsha 410128, Peoples R China;corresponding author), Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China;corresponding author), Guizhou Inst Technol, Coll Food & Pharmaceut Engn, Guiyang 550000, Peoples R China.|贵州理工学院食品药品制造工程学院;贵州理工学院;

年份：2022

卷号：27

期号：18

外文期刊名：MOLECULES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000856774700001)】；

基金：This work was finished in Key Laboratory of Crop Epigenetic Regulation and Development in Hunan Province, and supported by the Science and Technology Project of Hunan Province (No. 2014FJ3082).

语种：英文

外文关键词：alpha-galactosidase; Lactosphaera pasteurii; expression analysis; gene clone

摘要：alpha-Galactosidase (EC 3.2.1.22) refers to a group of enzymes that hydrolyze oligosaccharides containing oc-galactoside-banded glycosides, such as stachyose, raffinose, and verbascose. These enzymes also possess great potential for application in sugar production, and in the feed and pharmaceutical industries. In this study, a strain of Lactosphaera pasteurii (WHPC005) that produces oc-galactosidase was identified from the soil of Western Hunan, China. It was determined that the optimal temperature and pH for this oc-galactosidase were 45 degrees C and 5.5, respectively. The activity of oc-galactosidase was inhibited by K+, Al3+, Fe3+, fructose, sucrose, lactose, galactose, SDS, EDTA, NaCl, and (NH4)(2)SO4, and enhanced by Ca2+, Fe2+, mn(2), zn(2+), glucose, and raffinose. The optimal inducer was raffinose, and the optimal induction concentration was 30 mu mol/L. The alpha-galactosidase gene was cloned using random fragment cloning methods. Sequence analysis demonstrated that the open reading frame of the alpha-galactosidase gene was 1230 bp, which encodes a putative protein of 409 amino acids in length. Bioinformatics analysis showed that the isoelectric point and molecular weight of this alpha-galactosidase were 4.84 and 47.40 kD, respectively. Random coils, alpha helixes, and beta turns were observed in its secondary structure, and conserved regions were found in the tertiary structure of this alpha-galactosidase. Therefore, this alpha-galactosidase-producing bacterial strain has the potential for application in the feed industry.