文献类型：期刊文献

英文题名：Characterization and Genome Analysis of Cladobotryum mycophilum, the Causal Agent of Cobweb Disease of Morchella sextelata in China

作者：Liu, Zhenghui Cong, Yunlong Sossah, Frederick Leo Lu, Yongzhong Kang, Jichuan Li, Yu

第一作者：Liu, Zhenghui

通信作者：Kang, JC[1];Li, Y[2]

机构：[1]Guizhou Univ, Engn & Res Ctr Southwest Biopharmaceut Resources, Natl Educ Minist, Guiyang, Peoples R China;[2]Jilin Agr Univ, Engn Res Ctr Edible & Med Fungi, Minist Educ, Changchun 130118, Peoples R China;[3]Guizhou Univ, Res Inst Sci & Technol, Guiyang 550025, Peoples R China;[4]Oil Palm Res Inst, Council Sci & Ind Res CSIR, Coconut Res Programme, POB 245, Sekondi, Ghana;[5]Guizhou Inst Technol, Sch Food & Pharmaceut Engn, Guiyang 550003, Peoples R China

第一机构：Guizhou Univ, Engn & Res Ctr Southwest Biopharmaceut Resources, Natl Educ Minist, Guiyang, Peoples R China

通信机构：corresponding author), Guizhou Univ, Engn & Res Ctr Southwest Biopharmaceut Resources, Natl Educ Minist, Guiyang, Peoples R China;corresponding author), Jilin Agr Univ, Engn Res Ctr Edible & Med Fungi, Minist Educ, Changchun 130118, Peoples R China.

年份：2023

卷号：9

期号：4

外文期刊名：JOURNAL OF FUNGI

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000979261500001)】；

基金：This research was funded by the Guizhou Provincial Department of Education Young Science and Technology Talent Growth Project (grant No. Qian Jiao Ji [2022] 119); Guizhou University Natural Science Special (Special Post) Scientific Research Fund Project (grant No. Guida Tegang Hezi (2022) 17); Strategic Pilot Science and Technology Project of Chinese Academy of Sciences (grant No. XDA28080304); The National Key Research and Development Program of China (grant No. 2021YFD1600401); and The Natural Science Foundation of Guizhou Province (grant No. Qian Ke He Zhi Cheng (2021) Generally 200).

语种：英文

外文关键词：Morchella sextelata; cobweb disease; identification; Cladobotryum mycophilum; whole genome sequencing

摘要：Cobweb disease is a fungal disease that can cause serious damage to edible mushrooms worldwide. To investigate cobweb disease in Morchella sextelata in Guizhou Province, China, we isolated and purified the pathogen responsible for the disease. Through morphological and molecular identification and pathogenicity testing on infected M. sextelata, we identified Cladobotryum mycophilum as the cause of cobweb disease in this region. This is the first known occurrence of this pathogen causing cobweb disease in M. sextelata anywhere in the world. We then obtained the genome of C. mycophilum BJWN07 using the HiFi sequencing platform, resulting in a high-quality genome assembly with a size of 38.56 Mb, 10 contigs, and a GC content of 47.84%. We annotated 8428 protein-coding genes in the genome, including many secreted proteins, host interaction-related genes, and carbohydrate-active enzymes (CAZymes) related to the pathogenesis of the disease. Our findings shed new light on the pathogenesis of C. mycophilum and provide a theoretical basis for developing potential prevention and control strategies for cobweb disease.