文献类型：期刊文献

中文题名：HPLC法测定生姜半夏汤单煎与合煎中6-姜酚和6-姜醇含量

英文题名：Determination of 6-gingerol and 6-shogaol in ginger pinellia decoction of different decoction by HPLC

作者：刘芸 王珂佳 黄锴 莫正昌

第一作者：刘芸

机构：[1]铜仁职业技术学院药学院;[2]贵州轻工职业技术学院化工系;[3]贵州理工学院干部科

第一机构：铜仁职业技术学院药学院,贵州铜仁554300

年份：2015

卷号：0

期号：2

起止页码：168-170

中文期刊名：中国生化药物杂志

外文期刊名：Chinese Journal of Biochemical Pharmaceutics

语种：中文

中文关键词：生姜半夏汤;6-姜酚;单煎;合煎;6-姜醇;含量测定

外文关键词：ginger pinellia decoction ; 6-gingerol ; 6-shogaol ; single-decoction; mixed-decoction ; determination

摘要：目的 HPLC法测定生姜半夏汤单煎液与合煎液中6-姜酚、6-姜醇的含量变化。方法采用Amethyst C18-P柱(4.6 mm×250 mm,5μm),以甲醇-水(60∶40)为流动相,检测波长281 nm,流速0.9 m L/min,柱温25℃。结果 6-姜酚、6-姜醇在合煎液中含量均明显高于单煎液。结论生姜半夏汤使用时应采用合煎的方式制备,否则会降低6-姜酚、6-姜醇浸出的含量,影响生姜半夏汤的药效。
Objective To determine the content of 6-gingerol and 6-shogaol in separately decocted and mingly decocted ginger pinellia decoction by HPLC. Methods The samples were separated on Amethyst C18-P（ 4. 6 mm × 250 mm,5 μm） column. The mobile phase was methanol-water（ 60 ∶40） at the flow rate of 0. 9 m L / min. The detection wavelength was 281 nm. The column temperature was 25 ℃. Results The content of 6-gingerol and 6-shogaol in separately decocted obviously less than that in mingly decocted ginger pinellia decoction. Conclusion Ginger pinellia decoction should be used mingly decocted. Otherwise,it will reduce the contents of 6-gingerol and 6-shogaol,affect the efficacy of ginger pinellia decoction.