文献类型：期刊文献

英文题名：Isolation of Bacillus spp. with High Fibrinolytic Activity and Performance Evaluation in Fermented Douchi

作者：Long, Jia Zhang, Xin Gao, Zexin Yang, Yun Tian, Xueyi Lu, Mingyuan He, Laping Li, Cuiqin Zeng, Xuefeng

第一作者：Long, Jia

通信作者：He, LP[1];He, LP[2]

机构：[1]Guizhou Univ, Key Lab Agr & Anim Prod Storat & Proc Guizhou Pro, Guiyang 550025, Peoples R China;[2]Guizhou Univ, Coll Liquor & Food Engn, Guiyang 550025, Peoples R China;[3]Guizhou Univ, Sch Chem & Chem Engn, Guiyang 550025, Peoples R China;[4]GuiZhou Inst Technol, Coll Artificial Intelligence & Elect Engn, Guiyang 550003, Peoples R China

第一机构：Guizhou Univ, Key Lab Agr & Anim Prod Storat & Proc Guizhou Pro, Guiyang 550025, Peoples R China

通信机构：corresponding author), Guizhou Univ, Key Lab Agr & Anim Prod Storat & Proc Guizhou Pro, Guiyang 550025, Peoples R China;corresponding author), Guizhou Univ, Coll Liquor & Food Engn, Guiyang 550025, Peoples R China.

年份：2021

卷号：84

期号：4

起止页码：717-727

外文期刊名：JOURNAL OF FOOD PROTECTION

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000637289300021)】；

基金：This work was financially supported by the Key Agricultural Project of Guizhou Province (QKHZC-[2019]2382 and QKHZC-[2016]2580), National Natural Science Foundation of China (31660010 and 31870002), and Qiankehe talents project ([2018]5781 and [2017]5788-11).

语种：英文

外文关键词：Bacillus velezensis; Biological amine; Douchi; Fermentation performance; Fibrinolytic enzymes; Screening and identification

摘要：Fibrinolytic enzymes are effective and highly safe in treating cardiovascular and cerebrovascular diseases. Therefore, screening fibrinolytic enzyme-producing microbial strains with excellent fermentation performance is of great value to industrial applications. The fibrin plate method was used in screening strains with high yields of fibrinolytic enzymes from different fermented food products, and the screened strains were preliminarily identified using molecular biology. Then, the strains were used for solid-state fermentation of soybeans. Moreover, the fermentation product douchi was subjected to fibrinolytic activity measurement, sensory evaluation, and biogenic amine content determination. The fermentation performance of each strain was comprehensively evaluated through principal component analysis. Finally, the target strain was identified based on strain morphology, physiological and biochemical characteristics, 16S rDNA sequence, and phylogenetic analysis results. A total of 15 Bacillus species with high fibrinolysin activity were selected. Their fibrinolytic enzyme-producing activity levels were higher than 5,500 IU/g. Through molecular biology analysis, we found 4 strains of Bacillus subtilis, 10 strains of Bacillus amyloliquefaciens, and 1 strain of Bacillus velezensis. The principal component analysis results showed that SN-14 had the best fermentation performance and reduced the accumulation of histamine and total amine, the fibrinolytic activity of fermented douchi reached 5,920.5 +/- 107.7 IU/g, and the sensory score was 4.6 +/- 0.3 (out of 5 points). Finally, the combined results of physiological and biochemical analyses showed SN-14 was Bacillus velezensis. The high-yield fibrinolytic and excellent fermentation performance strain Bacillus velezensis SN-14 has potential industrial application.