文献类型：期刊文献

英文题名：Exogenous trehalose application promotes survival by alleviating oxidative stress and affecting transcriptome in ethanol-stressed Wickerhamomyces anomalus

作者：Li, Yinfeng Jiang, Guilan Long, Hua Liao, Yifa Huang, Mingzheng Yu, Zhihai Cheng, Shuang Wang, Ying Liu, Xiaozhu

第一作者：李宇芳

通信作者：Liu, XZ[1];Liu, XZ[2]|[14440df22f06bbcaad0e9]刘晓柱;

机构：[1]Guizhou Inst Technol, Guiyang, Peoples R China;[2]Nanyang Inst Technol, Nanyang, Peoples R China

第一机构：贵州理工学院

通信机构：corresponding author), Guizhou Inst Technol, Guiyang, Peoples R China;corresponding author), Nanyang Inst Technol, Nanyang, Peoples R China.|贵州理工学院;

年份：2023

外文期刊名：BRITISH FOOD JOURNAL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000989012800001)】；

基金：This study was supported by the National Natural Science Foundation of China (32160557) and Henan Key Laboratory of Industrial Microbial Resources and Fermentation Technology (HIMFT20210101).

语种：英文

外文关键词：Ethanol stress; Wickerhamomyces anomalus; Trehalose; Transcriptomics; Survival; Oxidative stress

摘要：PurposeIncreased ethanol accumulation during ethanol fermentation generates stress in yeast cells, which finally reduces the fermentation performance and efficiency. Trehalose, a potential stress protectant, has been reported to regulate the response of yeast to diverse environmental stresses. This study aimed to explore how exogenous trehalose application affects the survival, transcriptome and antioxidant enzymes of Wickerhamomyces anomalus grown under ethanol stress conditions.Design/methodology/approachExogenous trehalose was applied to the growth condition of W. anomalus, and optical densitometric method was used to detect contents of intracellular trehalose and MDA and activities of CAT and SOD. The survival was evaluated using spot analysis. Differentially expressed genes (DEGs) were identified through transcriptomics analysis.FindingsThe results showed that ethanol stress induced the accumulation of intracellular trehalose, with further exogenous trehalose application improving the survival and alleviating oxidative stress in ethanol-stressed W. anomalus. Transcriptomic results showed that trehalose has pleiotropic regulating effects on ethanol-stressed W. anomalus since most DEGs annotated to energy metabolism, amino acid metabolism, translation, folding, sorting and transport were affected post trehalose addition. Therefore, it is found that trehalose protected W. anomalus against ethanol stress, and these findings provide interesting insights into the mechanistic role of trehalose in improving ethanol stress tolerance of W. anomalus.Originality/value(1) Protective effect of exogenous trehalose addition on the survival of ethanol-stressed W. anomalus was proved. (2) Exogenous trehalose addition could partly alleviate oxidative stress induced by ethanol stress and affect transcriptome in W. anomalus.