文献类型：期刊文献

英文题名：Impact of Mild Field Drought on the Aroma Profile and Metabolic Pathways of Fresh Tea (Camellia sinensis) Leaves Using HS-GC-IMS and HS-SPME-GC-MS

作者：Liu, Xiaohui Dong, Fabao Li, Yucai Lu, Fu Wang, Botao Zhou, Taicen Zhao, Degang Huang, Mingzheng Wang, Feifei

第一作者：Liu, Xiaohui;刘晓辉

通信作者：Zhao, DG[1];Zhao, DG[2];Huang, MZ[3]

机构：[1]Guizhou Acad Agr Sci, Guizhou Inst Prataculture, Plant Conservat & Breeding Technol Ctr, Guizhou Key Lab Agr Biotechnol, Guiyang 550006, Peoples R China;[2]Guizhou Univ, Coll Tea Sci, Key Lab Plant Resources Conservat & Germplasm Inno, Minist Educ, Guiyang 550025, Peoples R China;[3]Guizhou Inst Technol, Coll Food & Pharmaceut Engn, Guiyang 550025, Peoples R China;[4]Yangzhou Univ, Inst Agr Sci, Jiangsu Coinnovat Ctr Modern Prod Technol Grain Cr, Key Lab Plant Funct Genom,Minist Educ,Jiangsu Key, Yangzhou 225009, Peoples R China

第一机构：Guizhou Acad Agr Sci, Guizhou Inst Prataculture, Plant Conservat & Breeding Technol Ctr, Guizhou Key Lab Agr Biotechnol, Guiyang 550006, Peoples R China

通信机构：corresponding author), Guizhou Acad Agr Sci, Guizhou Inst Prataculture, Plant Conservat & Breeding Technol Ctr, Guizhou Key Lab Agr Biotechnol, Guiyang 550006, Peoples R China;corresponding author), Guizhou Univ, Coll Tea Sci, Key Lab Plant Resources Conservat & Germplasm Inno, Minist Educ, Guiyang 550025, Peoples R China;corresponding author), Guizhou Inst Technol, Coll Food & Pharmaceut Engn, Guiyang 550025, Peoples R China.|贵州理工学院食品药品制造工程学院;贵州理工学院;

年份：2024

卷号：13

期号：21

外文期刊名：FOODS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001351329100001)】；

基金：This research was funded by the China Postdoctoral Science Foundation, grant number 2021M693807, the Science and Technology Plan Project of Guizhou province, grant number QKHJC-ZK[2022] YB180, the Open Fund Project of the Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region, Ministry of Education (Gui-zhou University), grant number QJH-KY-Z [2020]255, the Young Scientists Fund of the National Natural Science Foundation of China, grant number 32001463, Guizhou Fruit Wine Brewing Engineering Research Center, grant number [2022]050 and Guizhou Provincial Science and Technology Department, grant number KXJZ[2024]021. The APC was funded by the China Postdoctoral Science Foundation (2021M693807) and the Science and Technology Plan Project of Guizhou province, grant number QKHJC-ZK[2022] YB180.

语种：英文

外文关键词：Camellia sinensis cultivar; drought stress; fresh tea leaves; gas chromatography-ion mobility spectrometry (GC-IMS); headspace solid-phase micro-extraction gas chromatography-mass spectrometry (HS-SPME-GC-MS); aroma profile; odor activity values (OAVs); odor-active aroma compounds

摘要：Aroma plays a pivotal role in defining tea quality and distinctiveness, and tea producers have often observed that specific drought conditions are closely associated with the formation and accumulation of characteristic aroma compounds in tea leaves. However, there is still limited understanding of the differential strategies employed by various tea cultivars in response to drought stress for the accumulation of key volatile aroma compounds in fresh tea leaves, as well as the associated metabolic pathways involved in aroma formation. In this study, two widely cultivated tea cultivars in China, Fuding Dabai (FD) and Wuniuzao (WNZ), were examined to assess the impact of mild field drought stress on the composition and accumulation of key volatile aroma compounds in fresh leaves using headspace gas chromatography-ion mobility spectrometry (HS-GC-IMS) and headspace solid phase micro-extraction gas chromatography-mass spectrometry (HS-SPME-GC-MS) technologies. Results revealed that drought stress led to a substantial increase in the diversity of volatile compounds (VOCs) in FD, while WNZ exhibited a notable rise in low-threshold VOC concentrations, amplifying sweet, floral, fruity, and earthy aroma profiles in post-drought fresh leaves. Through partial least squares discriminant analysis (PLS-DA) of HS-GC-IMS and HS-SPME-GC-MS data, integrating variable importance projection (VIP) scores and odor activity values (OAVs) above 1, 9, and 13, key odor-active compounds were identified as potential markers distinguishing the drought responses in the two cultivars. These compounds serve as crucial indicators of the aromatic profile shifts induced by drought, providing insights into the differential metabolic strategies of the cultivars. Additionally, KEGG enrichment analysis revealed 12 metabolic pathways, such as terpenoid biosynthesis, fatty acid synthesis, cutin, suberine, and wax biosynthesis, and phenylalanine metabolism, which may play crucial roles in the formation and accumulation of VOCs in tea leaves under drought stress. These findings provide a comprehensive framework for understanding the cultivar-specific mechanisms of aroma formation and accumulation in tea leaves under mild drought conditions.