文献类型：期刊文献

英文题名：Transcriptional and Physiological Responses of Saccharomyces cerevisiae CZ to Octanoic Acid Stress

作者：Yu, Zhi-Hai Shi, Ming-Zhi Dong, Wen-Xuan Liu, Xiao-Zhu Tang, Wei-Yuan Huang, Ming-Zheng

第一作者：于志海

通信作者：Tang, WY[1];Huang, MZ[1]

机构：[1]Guizhou Inst Technol, Coll Food & Pharmaceut Engn, Guiyang 550003, Peoples R China

第一机构：贵州理工学院食品药品制造工程学院

通信机构：corresponding author), Guizhou Inst Technol, Coll Food & Pharmaceut Engn, Guiyang 550003, Peoples R China.|贵州理工学院食品药品制造工程学院;贵州理工学院;

年份：2025

卷号：11

期号：4

外文期刊名：FERMENTATION-BASEL

收录：;Scopus(收录号：2-s2.0-105003379378);WOS:【SCI-EXPANDED(收录号:WOS:001474969800001)】；

基金：This research was supported by Guizhou Provincial Basic Research Program (Natural Science), Grant/Award Number: Qiankehejichu MS[20259]195, Guizhou Fruit Wine Brewing Engineering Research Center (Qianjiaoji (2022)050), Guizhou Provincial Key Technology R&D Program (No. [2023] 069), Guizhou Provincial Science and Technology Department (KXJZ[2024]021), and Guizhou Provincial Science and Technology Foundation ([2024]512).

语种：英文

外文关键词：Saccharomyces cerevisiae; octanoic acid stress; growth inhibition; transcriptomic analysis; oxidative stress

摘要：This study elucidates the adaptive mechanisms of Saccharomyces cerevisiae CZ under octanoic acid stress, revealing concentration-dependent growth inhibition (76% lethality at 800 mg/L) and notable tolerance at 600 mg/L. Initial exposure (<= 6 h) showed no growth impairment, but prolonged treatment induced dose-dependent lethality, accompanied by reduced H+/K+-ATPase activity and elevated malondialdehyde (MDA) levels, indicative of oxidative damage. Transcriptomic profiling of 5665 genes highlighted the predominant downregulation of ribosomal functions (translation, ribosome biogenesis) and amino acid metabolism pathways (e.g., ARO10, ARO9). Strain-specific regulatory dynamics were observed: (1) TPO1-mediated efflux was active at 400 mg/L but absent at 600 mg/L, suggesting compensatory mechanisms under high stress; (2) HTX1-related genes exhibited bidirectional regulation (downregulated at 400 mg/L vs. upregulated at 600 mg/L), reflecting metabolic flexibility; (3) ACC1 downregulation (600 mg/L) and unaltered SFK1 expression contrasted with lipid-remodeling strategies in engineered strains; and (4) PMA2 suppression diverged from literature-reported PMA1 activation, underscoring strain-specific energy reallocation. Suppression of ergosterol biosynthesis and ribosomal genes revealed a trade-off between stress adaptation and biosynthetic processes. These findings reconcile prior contradictions by attributing discrepancies to genetic backgrounds (CZ vs. laboratory/engineered strains) and methodological variations. Unlike strains relying on phospholipid asymmetry or oleic acid overproduction, CZ's unique tolerance stems from integrated membrane homeostasis (via lipid balance) and metabolic conservation. This work emphasizes the critical role of strain-specific regulatory networks in octanoic acid resistance and provides insights for optimizing yeast robustness through targeted engineering of membrane stability and metabolic adaptability. Future studies should employ multi-omics integration to unravel the dynamic gene regulatory logic underlying these adaptive traits.