文献类型：期刊文献

英文题名：Protective effects of thiamine on Wickerhamomyces anomalus against ethanol stress

作者：Li, Yinfeng Long, Hua Jiang, Guilan Yu, Zhihai Huang, Mingzheng Zou, Shiping Guan, Tianbing Zhao, Yan Liu, Xiaozhu

第一作者：李宇芳

通信作者：Liu, XZ[1];Liu, XZ[2]|[14440df22f06bbcaad0e9]刘晓柱;

机构：[1]Guizhou Inst Technol, Guiyang, Peoples R China;[2]Chongqing Univ Sci & Technol, Chongqing Key Lab Ind Fermentat Microorganism, Chongqing, Peoples R China;[3]Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Peoples R China

第一机构：贵州理工学院

通信机构：corresponding author), Guizhou Inst Technol, Guiyang, Peoples R China;corresponding author), Chongqing Univ Sci & Technol, Chongqing Key Lab Ind Fermentat Microorganism, Chongqing, Peoples R China.|贵州理工学院;

年份：2022

卷号：13

外文期刊名：FRONTIERS IN MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000899360300001)】；

基金：Funding This study was supported by the National Natural Science Foundation of China (32160557) and Open Fund of Chongqing Key Laboratory of Industrial Fermentation Microorganism (Chongqing University of Science and Technology) (GYFJWSW-02).

语种：英文

外文关键词：ethanol stress; thiamine; Wickerhamomyces anomalus; transcriptomics; metabolomics

摘要：Wickerhamomyces anomalus (W. anomalus) is widely reported in the brewing industry and has positive effects on the aromatic profiles of wines because of its unique physiological characteristics and metabolic features. However, the accumulation of ethanol during fermentation inhibits the growth of W. anomalus. Thiamine is involved in the response against various abiotic stresses in microorganisms. Therefore, we used transcriptomic and metabolomic analyses to study the effect of thiamine on ethanol-stressed W. anomalus. The results indicate that thiamine could alleviate the inhibitory effect of ethanol stress on the survival of W. anomalus. Differentially expressed genes (DEGs) and differentially expressed metabolites (DEMs) caused by the thiamine intervention were identified as oxidative phosphorylation through integrated transcriptomic and metabolomic analyses. In addition, ethanol treatment decreased the content of intracellular adenosine triphosphate (ATP), while thiamine partially alleviated this phenomenon. The present comprehensive transcriptional overview and metabolomic analysis provide insights about the mechanisms of thiamine protection on W. anomalus under ethanol stress and promote the potential applications of W. anomalus in the fermentation industry.