文献类型：期刊文献

英文题名：Exogenous Thiamine Application Improves the Survival ofWickerhamomyces anomalusunder Ethanol Stress

作者：Li, Y. F. Jiang, G. L. Liao, Y. F. Long, H. Liu, X. Z.

第一作者：李银凤

通信作者：Liu, XZ[1]|[14440df22f06bbcaad0e9]刘晓柱;

机构：[1]Guizhou Inst Technol, Coll Food & Pharmaceut Engn, Guiyang 550003, Peoples R China

第一机构：贵州理工学院食品药品制造工程学院

通信机构：corresponding author), Guizhou Inst Technol, Coll Food & Pharmaceut Engn, Guiyang 550003, Peoples R China.|贵州理工学院食品药品制造工程学院;贵州理工学院;

年份：2023

卷号：92

期号：5

起止页码：658-665

外文期刊名：MICROBIOLOGY

收录：;Scopus(收录号：2-s2.0-85171423300);WOS:【SCI-EXPANDED(收录号:WOS:001070039300008)】；

基金：This study was supported by the National Natural Science Foundation of China (32 160 557)

语种：英文

外文关键词：Wickerhamomyces anomalus; ethanol stress; thiamine; survival; metabolomics

摘要：All living organisms require thiamine, a water-soluble vitamin, which is also reported to play a role in response to various abiotic stresses. However, the impact of thiamine on the growth of Wickerhamomyces anomalus under ethanol stress is still not completely understood. In this study, we investigated the effects of exogenous thiamine application on the growth of W. anomalus under ethanol stress. We also investigated how thiamine affected the differentially expressed metabolites (DEMs) using a non-targeted metabolomics approach. The results showed that exogenous thiamine application improved the growth of ethanol-stressed W. anomalus. The DEMs affected by exogenous thiamine application were significantly enriched as ABC transporters process, glycerophospholipid metabolism, purine metabolism, and one carbon pool via folate and sulfur relay system using Kyoto Encyclopedia of Genes and Genomics (KEGG) enrichment analysis. Several pathways, such as arginine biosynthesis, cell cycle, oxidative phosphorylation, etc., were also regulated by thiamine because they were not enriched after exogenous thiamine application. Therefore, thiamine helped to protect W. anomalus from the effects of ethanol stress. These findings offer a theoretical basis for further understanding the regulatory mechanism of thiamine-mediated ethanol stress tolerance in W. anomalus.