成果/Result
- Engineering of a chitosanase fused to a carbohydrate-binding module for continuous production of desirable chitooligosaccharides被引量：26收藏
- 作者：Zhou, Jianli Harindintwali, Jean Damascene Yang, Wenhua Han, Minghai Deng, Bin
- 机构：Jiangnan Univ;Guizhou Inst Technol;Guangdong Tech Israel Inst Technol
- 来源：CARBOHYDRATE POLYMERS 2021
- 关键词：Bioproduction Chitooligosaccharide Chitosanase Carbohydrate-binding module One-step purification and immobilization Packed-bed reactor
- Activation of the Unfolded Protein Response via Co-expression of the HAC1(i) Gene Enhances Expression of Recombinant Elastase in Pichia pastoris被引量：10收藏
- 作者：Han, Minghai Wang, Weixian Zhou, Jianli Gong, Xun Xu, Cunbin
- 机构：Guizhou Inst Technol;Guangdong Prov Key Lab Fermentat & Enzyme Engn;Jiangnan Univ
- 来源：BIOTECHNOLOGY AND BIOPROCESS ENGINEERING 2020
- 关键词：unfolded protein response Pichia pastoris protein expression HAC1
- Enhancement of the performance of the GH75 family chitosanases by fusing a carbohydrate binding module and insights into their substrate binding mechanisms被引量：9收藏
- 作者：Zhou, Jianli Gu, Qiuya Shen, Yu Harindintwali, Jean Damascene Yang, Wenhua
- 机构：Guizhou Univ;Jiangnan Univ;Jiangnan Univ;Guizhou Inst Technol;Univ Chinese Acad Sci
- 来源：LWT-FOOD SCIENCE AND TECHNOLOGY 2022
- 关键词：Chitosan Chitooligosaccharide Chitosanase Carbohydrate-binding module Thermodynamic analysis
- A modified method of gene disruption in Komagataella phaffii with Cre/ loxP system被引量：3收藏
- 作者：Han, Minghai Wang, Weixian Gong, Xun Zhu, Guofei Liu, Xiaohui
- 机构：Guizhou Inst Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2022
- 关键词：Komagataella phaffii Pichia pastoris Gene disruption Marker rescue Cre loxP
- Increased Expression of Recombinant Chitosanase by Co-expression of Hac1p in the Yeast Pichia pastoris被引量：2收藏
- 作者：Han, Minghai Wang, Weixian Gong, Xun Zhou, Jianli Xu, Cunbin
- 机构：Guizhou Inst Technol;South China Normal Univ
- 来源：PROTEIN AND PEPTIDE LETTERS 2021
- 关键词：Unfolded protein response Hac1p Pichia pastoris chitosanase secretory expression co-expression
- Recycling selectable markers via Cre/loxP system for constructing Komagataella phaffii strains co-expressing multiple proteins被引量：0收藏
- 作者：Wang, Weixian Han, Minghai Zhu, Guofei Liu, Xiaohui Zhao, Tianming
- 机构：Guizhou Inst Technol;Guizhou Inst Technol
- 来源：BIOTECHNOLOGY LETTERS 2024
- 关键词：Cre/loxP Co-express multi-protein Komagataella phaffii Pichia pastoris Selectable marker
