文献类型：期刊文献

英文题名：Analysis of the ethanol stress response mechanism in Wickerhamomyces anomalus based on transcriptomics and metabolomics approaches

作者：Li, Yinfeng Long, Hua Jiang, Guilan Gong, Xun Yu, Zhihai Huang, Mingzheng Guan, Tianbing Guan, Yuanyuan Liu, Xiaozhu

第一作者：李宇芳

通信作者：Liu, XZ[1];Liu, XZ[2]|[14440df22f06bbcaad0e9]刘晓柱;

机构：[1]Guizhou Inst Technol, Guiyang 550000, Peoples R China;[2]Chongqing Univ Sci & Technol, Chongqing 404100, Peoples R China;[3]Henan Inst Sci & Technol, Xinxiang 453000, Henan, Peoples R China

第一机构：贵州理工学院

通信机构：corresponding author), Guizhou Inst Technol, Guiyang 550000, Peoples R China;corresponding author), Chongqing Univ Sci & Technol, Chongqing 404100, Peoples R China.|贵州理工学院;

年份：2022

卷号：22

期号：1

外文期刊名：BMC MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000884883200001)】；

基金：This study was supported by the National Natural Science Foundation of China (32160557), and Open Fund of Chongqing Key Laboratory of Industrial Fermentation Microorganism (Chongqing University of Science and Technology) (GYFJWSW-02).

语种：英文

外文关键词：Ethanol stress; Growth; W; anomalus; Transcriptomics; Metabolomics

摘要：Background Wickerhamomyces anomalus (W. anomalus) is a kind of non-Saccharomyces yeast that has a variety of unique physiological characteristics and metabolic features and is widely used in many fields, such as food preservation, biomass energy, and aquaculture feed protein production. However, the mechanism of W. anomalus response to ethanol stress is still unclear, which greatly limits its application in the production of ethanol beverages and ethanol fuels. Therefore, we checked the effects of ethanol stress on the morphology, the growth, and differentially expressed genes (DEGs) and metabolites (DEMs) of W. anomalus. Results High concentrations of ethanol (9% ethanol and 12% ethanol) remarkably inhibited the growth of W. anomalus. Energy metabolism, amino acid metabolism, fatty acids metabolism, and nucleic acid metabolism were significantly influenced when exposing to 9% ethanol and 12% ethanolstress, which maybe universal for W. anomalus to response to different concentrations of ethanol stressl Furthermore, extracellular addition of aspartate, glutamate, and arginine significantly abated ethanol damage and improved the survival rate of W. anomalus. Conclusions The results obtained in this study provide insights into the mechanisms involved in W. anomalus response to ethanol stress. Therefore, new strategies can be realized to improve the ethanol tolerance of W. anomalus through metabolic engineering.