文献类型：期刊文献

英文题名：Transcriptomic Analysis of Cell Stress Response in Wickerhamomyces anomalus H4 Under Octanoic Acid Stress

作者：Yu, Zhi-Hai Li, Li Chen, Qiu-Yu Huang, Bing-Xuan Shi, Ming-Zhi Dong, Wan-Jin Zu, Yuan Huang, Ming-Zheng Liu, Xiao-Zhu

第一作者：于志海

通信作者：Yu, ZH[1]

机构：[1]Guizhou Inst Technol, Coll Food & Pharmaceut Engn, Guiyang 550003, Peoples R China

第一机构：贵州理工学院食品药品制造工程学院

通信机构：corresponding author), Guizhou Inst Technol, Coll Food & Pharmaceut Engn, Guiyang 550003, Peoples R China.|贵州理工学院食品药品制造工程学院;贵州理工学院;

年份：2024

卷号：10

期号：11

外文期刊名：FERMENTATION-BASEL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001365034000001)】；

基金：This research was funded by Guizhou Fruit Wine Brewing Engineering Research Center (Qianjiaoji (2022)050), Guizhou Provincial Key Technology R&D Program (No. [2023] 069), and Guizhou Provincial Science and Technology Department (KXJZ[2024]021).

语种：英文

外文关键词：Wickerhamomyces anomalus H4; stress tolerance; transcriptomics; octanoic acid; brewing and fermentation

摘要：The purified yeast strain H4, identified as W. anomalus through morphological, genetic, and phylogenetic analyses, was characterized and compared to a commercial Saccharomyces cerevisiae strain X16. W. anomalus H4 exhibited distinct morphological features. It demonstrated notable tolerance to 11% ethanol, 220 g/L glucose, and 200 mg/L octanoic acid, similar to X16, except for having a lower tolerance to SO2. Survival analysis under various stress conditions revealed that ethanol and octanoic acid had the most detrimental effects, with 56% cell mortality at 13% ethanol and 400 mg/L octanoic acid. Transcriptomic analysis under octanoic acid stress showed that at 200 mg/L, 3369 differentially expressed genes (DEGs) were induced, with 1609 being upregulated and 1760 downregulated, indicating broad transcriptional reprogramming. At 400 mg/L, only 130 DEGs were detected, suggesting a more limited response. KEGG pathway analysis indicated that most DEGs at 200 mg/L were associated with the "ribosome" and "proteasome" pathways, reflecting disruptions in protein synthesis and turnover. At 400 mg/L, the DEGs were primarily related to "DNA replication" and "pyruvate metabolism". These findings highlight the adaptive mechanisms of W. anomalus H4 to environmental stresses, particularly octanoic acid, and its potential for use in brewing and fermentation processes.