文献类型：期刊文献

英文题名：Whole-Transcriptome Sequencing Reveals a ceRNA Regulatory Network Associated with the Process of Periodic Albinism under Low Temperature in Baiye No. 1 (Camellia sinensis)

作者：Xu, Cunbin Li, Jinling Wang, Hualei Liu, Huijuan Yu, Zhihai Zhao, Zhi

第一作者：许存宾;Xu, Cunbin

通信作者：Zhao, Z[1]

机构：[1]Guizhou Univ, Coll Life Sci, Guiyang 550025, Peoples R China;[2]Guizhou Univ, Guizhou Key Lab Propagat & Cultivat Med Plants, Guiyang 550025, Peoples R China;[3]Guizhou Inst Technol, Coll Food & Pharmaceut Engn, Guiyang 550003, Peoples R China

第一机构：Guizhou Univ, Coll Life Sci, Guiyang 550025, Peoples R China

通信机构：corresponding author), Guizhou Univ, Guizhou Key Lab Propagat & Cultivat Med Plants, Guiyang 550025, Peoples R China.

年份：2023

卷号：24

期号：8

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000977594600001)】；

语种：英文

外文关键词：Camellia sinensis; Baiye No; 1; periodic albinism; whole transcriptome; ceRNA; non-coding RNA

摘要：The young shoots of the tea plant Baiye No. 1 display an albino phenotype in the early spring under low environmental temperatures, and the leaves re-green like those of common tea cultivars during the warm season. Periodic albinism is precisely regulated by a complex gene network that leads to metabolic differences and enhances the nutritional value of tea leaves. Here, we identified messenger RNAs (mRNAs), long noncoding RNAs (lncRNAs), circular RNAs (circRNAs), and microRNAs (miRNAs) to construct competing endogenous RNA (ceRNA) regulatory networks. We performed whole-transcriptome sequencing of 12 samples from four periods (Bud, leaves not expanded; Alb, albino leaves; Med, re-greening leaves; and Gre, green leaves) and identified a total of 6325 differentially expressed mRNAs (DEmRNAs), 667 differentially expressed miRNAs (DEmiRNAs), 1702 differentially expressed lncRNAs (DElncRNAs), and 122 differentially expressed circRNAs (DEcircRNAs). Furthermore, we constructed ceRNA networks on the basis of co-differential expression analyses which comprised 112, 35, 38, and 15 DEmRNAs, DEmiRNAs, DElncRNAs, and DEcircRNAs, respectively. Based on the regulatory networks, we identified important genes and their interactions with lncRNAs, circRNAs, and miRNAs during periodic albinism, including the ceRNA regulatory network centered on miR5021x, the GAMYB-miR159-lncRNA regulatory network, and the NAC035-miR319x-circRNA regulatory network. These regulatory networks might be involved in the response to cold stress, photosynthesis, chlorophyll synthesis, amino acid synthesis, and flavonoid accumulation. Our findings provide novel insights into ceRNA regulatory mechanisms involved in Baiye No. 1 during periodic albinism and will aid future studies of the molecular mechanisms underlying albinism mutants.